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本文应用核酸分子杂交技术对喉癌组织中Ｃ－ｍｙｃ癌基因扩增进行了研究，结果发现：①喉癌组织中具有Ｃ－ｍｙｃ癌基因扩增，其平均扩增倍数为正常喉组织的２．３倍。②Ｃ－ｍｙｃ癌基因扩增水平与喉癌临床分期有关。表现为Ｎ期肿瘤Ｃ－ｍｙｃ基因扩增明显高于Ｉ期和Ⅱ期，Ⅲ期又明显高于１期。本研究结果表明：在喉癌组织中Ｃ－ｍｙｃ基因以扩增形式被激活，激活后的癌基因对喉癌的发生、发展可能起着重要的作用；Ｃ－ｍｙｃ基因扩增与喉癌发展阶段相关，其扩增程度随着肿瘤的病期增高而增高，揭示Ｃ－ｍｙｃ癌基因扩增水平有可能作为肿瘤病情进展和恶性度的指标。本实验从基因水平上探讨了喉癌组织中Ｃ－ｍｙｃ癌基因的激活方式，为今后进一步研究喉癌发生发展机理以及对肿瘤进行基因诊断、治疗提供了实验依据．
In this paper, we studied the amplification of C-myc oncogene in laryngeal carcinoma using nucleic acid hybridization. The results showed that: (1) C-myc oncogene was amplified in laryngeal carcinoma with the average multiplication ratio of 2 .3 times. ② The level of C-myc oncogene amplification correlated with the clinical stage of laryngeal cancer. The expression of C-myc gene in stage N was significantly higher than that in stage I and II, and was significantly higher than that in stage III. The results of this study show that: C-myc gene is activated in the form of amplification in laryngeal carcinoma, the activated oncogene may play an important role in the occurrence and development of laryngeal carcinoma; C-myc gene amplification and laryngeal cancer development Stage, the extent of its expansion with the tumor stage increased and increased, revealing the level of C-myc oncogene amplification may be used as an indicator of tumor progression and malignancy. This experiment from the gene level to explore the activation of C-myc oncogene in laryngeal cancer tissue for the future further study of the mechanism of development and progression of laryngeal cancer gene diagnosis and treatment provide experimental evidence.