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目的:建立高效液相色谱法测定大鼠血浆中来那度胺的浓度。方法:色谱柱采用VenusilASBC18column(4.6 mm×250mm,5μm)购自于博纳艾杰尔科技有限公司。样品采用梯度洗脱,流动相为乙腈和0.05%甲酸溶液,流速为1.0 mL/min,检测波长为254 nm,以沙利度胺为内标。结果:来那度胺血药浓度的线性范围为0.1-5μg/mL,最低检测限为0.1μg/mL,三个浓度的QC样品(0.2,1 and 5μg/mL)的提取回收率分别为78.8±3.8,80.1±3.2 and 79.1±7.6%。结论:此方法准确、简便、灵敏度高,对来那度胺的血药浓度测定和药物代谢动力学研究极具价值。
Objective: To establish a method for the determination of lenalidomide in rat plasma by high performance liquid chromatography. Methods: The column was purchased from Bonaventure AG with Venusil ASBC18column (4.6 mm × 250 mm, 5 μm). The sample was eluted with a gradient of acetonitrile and 0.05% formic acid at a flow rate of 1.0 mL / min with a detection wavelength of 254 nm and an internal standard of thalidomide. Results: The linear range of lenalidomide plasma concentration was 0.1-5μg / mL with the detection limit of 0.1μg / mL. The recovery rates of three concentrations of QC samples (0.2,1 and 5μg / mL) were 78.8 ± 3.8, 80.1 ± 3.2 and 79.1 ± 7.6%. Conclusion: This method is accurate, simple and sensitive. It is very valuable for the determination of plasma concentration and pharmacokinetics of lenalidomide.