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为观察模拟失重大鼠延髓 FOS表达以及脊髓交感节前神经元是否发生一定改变 ,本研究采用尾部悬吊大鼠模型模拟失重影响 ,用抗 FOS蛋白和抗酪氨酸羟化酶 ( TH)双重免疫组织化学反应和 Nissl染色法 ,观察了 4周的在模拟失重下延髓 FOS蛋白表达与 TH阳性神经元的关系以及脊髓 C8、T1侧角细胞大小的改变 ,并与对照大鼠进行比较。结果显示 :与对照组大鼠比较 ,模拟失重大鼠出现了以下的改变 :( 1) FOS蛋白表达主要局限在延髓内脏带 ( MVZ)区 ,并以背内侧的孤束核与腹外侧区较为密集 ,并且发现有近 3 0 %的 TH神经元出现 FOS表达 ;( 2 )三叉神经脊束核尾侧亚核和薄束核亦出现 FOS表达 ;( 3 ) C8、T1侧角细胞胞体增大。本研究提示 ,在 4周模拟失重条件下 MVZ可能参与失重状态下心血管适应性变化的中枢调控 ,且儿茶酚胺能神经元参与这种作用的调节 ;脊髓侧角细胞发生代偿性增大变化。本文还就这种变化在航天飞行后心血管失调发生机理中的意义进行了讨论
In order to observe whether the FOS expression in the medulla oblongata and the preganglionic neurons in the spinal cord mimic some changes, this study used tail-suspended rat model to simulate the effects of weightlessness on the expression of anti-FOS protein and anti-tyrosine hydroxylase (TH) Immunohistochemistry and Nissl staining were used to observe the relationship between FOS protein expression and TH positive neurons in the medulla oblongata under simulated weightlessness and changes in the size of the horn cells on the C8 and T1 sides of the spinal cord at four weeks. The results showed that compared with the control group, the following changes were observed in the simulated weightless rats: (1) The expression of FOS protein was mainly localized in the medullary visceral zone (MVZ), and the dorsal to the medial solitary nucleus and ventrolateral region Dense, and found that nearly 30% of TH neurons appear FOS expression; (2) the trigeminal nucleus caudalis nucleus and the bundle of nuclear also appear FOS expression; (3) C8, T1 cell body horn cells increased . This study suggests that under simulated weightlessness for 4 weeks, MVZ may participate in the central regulation of cardiovascular adaptive changes in weightlessness, and catecholaminergic neurons are involved in the regulation of this effect. The compensatory increase of spinal cord angle cells occurs. The paper also discusses the significance of this change in the pathogenesis of cardiovascular disorders after space flight