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我们于1974年开始水稻联合共生固氮的探讨,四年来先后在福建的晋江、龙海等13个县市,采集了69个水稻品种根系的样品226个,使用乙炔还原法测定根系的固氮活性和进行水稻根内固氮菌的分离。通过测定水稻根表灭菌与不灭菌、根表灭菌后加培养基增菌培养以及延长增菌培养时间后的根系的固氮酶活性,初步说明了水稻根内存在固氮菌。在所测定的田间和盆栽水稻根系中有71.4—83.7％的水稻根样的内部存在固氮菌(有固氮酶活性)。使用的增菌培养基是慢型快长根瘤菌培养基及苹果酸钠H培养基。根据培养基的特点和分离菌的营养要求,根内固氮菌同自生固氮根瘤菌相似,而不同于自生固氮菌。
In 1974, we discussed the symbiotic nitrogen fixation of rice. In the past four years, we collected 226 root samples from 69 rice cultivars in 13 counties and cities such as Jinjiang and Longhai in Fujian Province. The nitrogen fixation activity of root system was measured by acetylene reduction method. Nitrogen-fixing bacteria in rice roots were isolated. Nitrogen-fixing bacteria were preliminarily identified in the roots of rice by measuring the sterilizing and non-sterilizing of the rice root surface, enriching the medium with the sterilized sterilized root surface and prolonging the activity of nitrogenase in the roots after the enrichment culture. Azotobacteria (with nitrogenase activity) were found in the rice roots of 71.4-83.7% of the rice root samples tested in the field and potted rice. The enrichment medium used is a slow-type fast root nodule culture medium and sodium malate H medium. According to the characteristics of the culture medium and the nutritional requirements of the isolates, the nitrogen-fixing bacteria in the root are similar to the autotrophic nitrogen-fixing rhizobia, but different from the autotrophic nitrogen-fixing bacteria.