场流分级是一种分离方法,在处理大分子和柱子混合物方面它具有一定的优越性。本质上场流分级是一种单相色谱,用外场或梯度代替原来的固定相。本文介绍了场流分级的基本原理和一些分支技术并例举了这些方法对大分子和细粒子的应用。 Field-flow classification is a separation method that has certain advantages in handling macromolecules and column mixtures. In essence, field-flow fractionation is a single-phase chromatography, using an external field or gradient instead of the original stationary phase. This paper introduces the basic principles of field-flow classification and some branching techniques and illustrates the application of these methods to macromolecules and fine particles.