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稻瘟病是影响水稻高产、稳产、优质的重要病害。广谱持久抗瘟品种的培育和利用是防治稻瘟病最经济有效的措施之一,但广谱持久抗性分子机制还不清楚。子预44是一具有广谱持久稻瘟病抗性的云南地方粳稻品种,对其抗瘟基因的鉴定将有助于揭示其抗瘟分子机制。本研究通过利用子预44和感病水稻江南香糯杂交构建F2遗传群体,稻瘟病菌株LP33苗期喷雾接种亲本预44、江南香糯及F2代单株,对其抗性进行评价和主效抗瘟基因定位。研究结果显示:子预44表现高抗,江南香糯高感;F2群体稻瘟病抗感分离符合3:1的分离比,即子预44对LP33的抗性为单显性基因控制的抗性遗传,并将该基因暂定名为Pi-zy3(t)。进一步利用SSR分子标记将Pi-zy3(t)定位在水稻第六号染色体RM276到RM3827之间,为进一步的基因克隆和抗病机分子制研究奠定了基础,同时也为利用子预44进行抗病分子育种提供了辅助选择的分子标记。
Rice blast is an important disease that affects high yield, stable yield and high quality of rice. The cultivation and utilization of a long-lasting, broad-spectrum anti-blast variety is one of the most economical and effective measures for preventing and controlling blast, but the mechanism of the broad-spectrum persistent resistance is not yet clear. Sub-pre-44 is a local japonica rice variety with long-lasting resistance to blast in Yunnan. Identification of its blast resistance genes will help reveal its molecular mechanism of anti-blast. In this study, the F2 population was constructed by crossing the susceptible rice Jiangnan Xiangnuo with the pre-44 and the susceptible rice cultivars were pre-44, Jiangnan-Xiangnuo and F2, and their resistance was evaluated by spraying Anti-blast gene mapping. The results showed that sub-pre-44 exhibited high resistance and high susceptibility to Xiangnuo in Jiangnan. The anti-susceptibility separation of F2 population was consistent with the segregation ratio of 3: 1, that is, the resistance of pre-44 to LP33 was controlled by single dominant gene The gene was tentatively named Pi-zy3 (t). Further, SSR markers were used to locate Pi-zy3 (t) between RM276 and RM3827 on chromosome 6, which laid the foundation for further gene cloning and molecular pathogenesis research. At the same time, Disease molecular breeding provides molecular markers that aid selection.