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目的筛选医院呼吸科环境中分枝杆菌污染检测的适宜方法,为防控分枝杆菌医院感染提供参考依据。方法采用平板沉降法和微生物采样方法对江苏省某医院呼吸科环境空气和物体表面以及医疗器械进行采样监测与细菌分离鉴定。结果用平板沉降法和采样器以及气溶胶法共计采集空气样本52份,通过细菌分离培养法均未检出分枝杆菌。用棉拭子涂抹法采集气空调滤网、物体表面和支气管镜等样本共计16份,细菌分离培养也未检出分枝杆菌。气溶胶法采集的空气样本和空调滤网样本,采用线性探针基因杂交技术检测,分枝杆菌基因检出率达50.0%。结论采用常规微生物采样和细菌培养检测方法,在医院呼吸科空气、物体表面和支气管镜等样本中均未检出分枝杆菌,用线性探针基因杂交技术在空气和空调滤网上检出分枝杆菌基因。
Objective To screen suitable methods for the detection of Mycobacterium tuberculosis in the respiratory department of the hospital and provide a reference for prevention and control of mycobacterial nosocomial infections. Methods Sampling monitoring and bacterial isolation and identification of the ambient air, the surface of the objects and the medical devices of the respiratory department of a hospital in Jiangsu Province were carried out by the method of plate settlement and microbiological sampling. Results A total of 52 air samples were collected by flat sedimentation method, sampler and aerosol method. Mycobacteria were not detected by bacterial isolation and culture method. A cotton swab smear method was used to collect air-conditioning filter, the surface of the object and a total of 16 samples of bronchoscopy, bacterial isolation and culture did not detect mycobacteria. The air samples collected by aerosol method and air-conditioning filter samples were detected by linear probe gene hybridization. The detection rate of mycobacteria reached 50.0%. Conclusion Mycobacterium was not detected in the air samples of the respiratory department of the hospital, the surface of the body and bronchoscope by routine microbiological sampling and bacterial culture detection. Branches were detected on the air and air-conditioning filter by linear probe gene hybridization Bacillus genes.