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目的: 为监测脊髓灰质炎 (以下简称脊灰) 高危人群健康儿童中, 是否还有脊灰野病毒株循环。方法: 在曾是脊灰流行区的乡镇, 于1997～1998 年定点采集0～4 岁健康儿童粪便计538 人份, 用Hep-2C细胞分离病毒; 鉴定为脊灰病毒, 采用温度特征试验作毒力测定和RT-PCR试验型内分析。结果: 在2 年的监测中, 计分离到脊灰Ⅰ型病毒10 株, Ⅲ型病毒3株, 非脊髓灰质炎肠道病毒12 株。分离的脊灰病毒温度特征试验, 两种温度滴度差均> 5, 判为弱毒(疫苗) 株; RT-PCR型内分析亦为疫苗(Sabin) 相关株, 两试验结果吻合。结论: 在具有高免疫屏障的健康儿童中, 仍有脊灰病毒循环, 但均为疫苗相关株
Purpose: To monitor the circulating of wild poliovirus strains in healthy children who are at high risk for poliomyelitis (hereinafter referred to as polio). Methods: In villages and towns that were once endemic to poliomyelitis, 538 feces were collected from 0 to 4 years-old children from 1997 to 1998, and the virus was isolated from Hep-2C cells. The virus was identified as poliovirus. Toxicity determination and RT-PCR assay type analysis. Results: In 2 years of surveillance, 10 strains of poliovirus type I, 3 strains of type III and 12 strains of non-polio enterovirus were isolated and counted. The temperature characteristic test of the isolated poliovirus showed that the titer difference between the two kinds of temperature was> 5, which was judged to be the attenuated (vaccine) strain. The RT-PCR type analysis was also related to the vaccine strain (Sabin). CONCLUSION: Poliovirus is still circulating in healthy children with high immune barrier, but all are vaccine-associated strains