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目的:研究丹红注射液对大鼠背部超长随意皮瓣存活的影响。方法:采用改良大鼠“Mc Farlane flap”模型,将实验动物随机分成实验组(丹红注射液组)和生理盐水对照组(生理盐水组),实验组腹腔注射2.5 ml/kg丹红注射液,生理盐水组注射2.5 m L/kg生理盐水,连续注射7 d后分别检测两组皮瓣存活面积比,并取皮瓣组织做光镜观察,免疫组化检测血管内皮生长因子(VEGF)表达,试剂盒检测超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果:术后7 d,实验组皮瓣的存活面积比为(80.61±5.56)%,对照组皮瓣的存活面积比为(48.78±4.34)%,差异有统计学意义(P<0.01)。实验组皮瓣坏死与存活并存的中区血管化程度,VEGF的表达均高于对照组。实验组较对照组皮瓣中SOD显著升高,MDA显著降低。结论:丹红注射液可能通过刺激VEGF表达,促进皮瓣新生血管增生,改善皮瓣血供,降低缺血再灌注损伤,从而提高大鼠随意皮瓣的存活。
Objective: To study the effect of Danhong Injection on the survival of the super long random flaps in rats. Methods: The experimental rats were randomly divided into experimental group (Danhong injection group) and saline control group (normal saline group) by using modified rat model of “McFarlane flap”. The experimental group was injected intraperitoneally with 2.5 ml / kg Danhong The rats were injected with 2.5 m L / kg normal saline at a dose of 2.5 m L / kg normal saline for 7 days. The survival ratio of the two groups was detected by light microscopy. The expression of vascular endothelial growth factor (VEGF) ). The kit was used to detect the content of superoxide dismutase (SOD) and malondialdehyde (MDA). Results: At 7 days after operation, the survival ratio of the flaps in the experimental group was (80.61 ± 5.56)%, while the survival rate of the flaps in the control group was (48.78 ± 4.34)%, the difference was statistically significant (P <0.01). In the experimental group, the degree of vascularization and the expression of VEGF in the necrosis and survival of the flaps were higher than those in the control group. The SOD in the experimental group was significantly higher than that in the control group, MDA was significantly decreased. CONCLUSION: Danhong injection may improve the survival of the random flap in rats by stimulating the expression of VEGF, promoting the angiogenesis of the flap, improving the blood supply of the flap and reducing the ischemia-reperfusion injury.