论文部分内容阅读
对大鲵(Andrias davidianus)2个驯化种群的核DNA进行PCR扩增,获得大鲵核DNA上编码核糖体5.8S rRNA和28S rRNA基因的部分序列和完整的ITS2序列(606 bp)。运用DNA分析软件对大鲵2个驯养种群(重庆水产研究所长寿湖珍稀鱼类繁育中心及广汉珍稀鱼类养殖公司)进行了遗传多样性分析。结果显示,该序列平均T、C、A、G含量分别为15.6%、36.5%、12.5%和35.4%,其中G、C的含量C+G(平均为71.9%)显著高于A、T含量A+T(平均为28.1%)。所测序列中有271个碱基发生颠换,112个碱基发生转换,转换与颠换比值(Si/Sv)为0.41,颠换大于转换。10个体均为单倍型,单倍型多样度(H)均为1.000 0±0.126 0,平均核苷酸差异系数(K)为5.932 1±0.861 4,核苷酸多样性(Pi)为0.631 9±0.013 9。种群遗传分化度(Fst)为0.030 5,中性检验及聚类分析表明2个群体没有分化成单一的群体,2个驯养种群遗传多样性好。
The nuclear DNAs of two domestication populations of Andrias davidianus were amplified by PCR. The partial sequences of ribosomal 5.8S rRNA and 28S rRNA genes and the complete ITS2 sequence (606 bp) were obtained. DNA analysis software was used to analyze the genetic diversity of two domesticated populations (Longevity Lake Rare Fish Breeding Center of Chongqing Fisheries Research Institute and Guanghan Rare Fish Culture Company). The results showed that the contents of T, C, A and G in the sequence were 15.6%, 36.5%, 12.5% and 35.4%, respectively. The content of G and C in C + G was 71.9% A + T (averaging 28.1%). In the sequence tested, 271 bases were transposed and 112 bases were transposed. The conversion / transversion ratio (Si / Sv) was 0.41, and the transversion was larger than the conversion. 10 individuals were all haplotype. The haplotype diversity (H) was 1.000 0 ± 0.126 0, the average nucleotide difference coefficient (K) was 5.932 1 ± 0.861 4, and the nucleotide diversity (Pi) was 0.631 9 ± 0.013 9. The genetic differentiation degree (Fst) of the population was 0.030 5. Neutrality test and cluster analysis showed that the two groups did not differentiate into a single population, and the two domesticated populations had good genetic diversity.