快速高效的DNA提取方法是玉米大规模分子育种的第一步,因此,需要发展一种快速提取玉米基因组DNA的方法,以满足我国中小型实验室的需要。本研究介绍了一种可用于玉米大规模基因组DNA提取的方法:先利用改良碱煮法提取玉米籽粒小块胚乳的DNA,进行基因型鉴定,对大群体进行筛选,再利用电钻磨样及简化的CTAB法快速提取玉米苗期叶片的DNA,进行后期的研究。这种方法使得玉米大群体的筛选和研究简单化、快速化、常规化,可广泛应用于玉米分子育种。 The rapid and efficient DNA extraction method is the first step of large-scale molecular breeding in maize. Therefore, there is a need to develop a method for rapid extraction of maize genomic DNA to meet the needs of our country’s small and medium sized laboratories. This study introduced a method for large-scale genomic DNA extraction in maize. Firstly, the DNA of small endosperm of maize kernels was extracted by modified alkali-boiled method, and then the genotypes were identified and large groups were screened. CTAB method to quickly extract the leaves of corn seedling DNA for later study. This method makes large maize screening and research simple, rapid, routine, can be widely used in maize molecular breeding.