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目的 分析GSTP1基因型与肺癌易感性的关系 ,探讨肺癌的敏感基因型 ,为肺癌易感者的筛检和肺癌病人的早期诊断提供依据 ,作好肺癌的二级预防措施。方法 应用病例对照研究方法与基因体外扩增限制性片段长度多态性分析技术相结合的分子流行病学研究 ,检测GSTP1基因型的分布频率 ,分析GSTP1基因多态性与肺癌易感性的关系 ,评价GSTP1突变基因型对肺癌发病的影响。结果 分析 32 6例GSTP1基因多态性 ,其中病例组 15 8例 ,对照组16 8例。总体上病例组与对照组的AA、AG和GG基因型的分布频率的差异无显著的统计学意义 ;5 0岁以上不吸烟的男性、含有GSTP1突变基因型 (AG或GG)者在病例组和对照组中的分布频率的差异有非常显著的统计学意义 ;GSTP1突变基因型与吸烟对肺癌的发生不存在交互作用。结论 5 0岁以上的男性、含有GSTP1突变基因型的人患肺癌的危险性比不合GSTP1突变基因型者大 8 3倍
Objective To analyze the relationship between GSTP1 genotypes and the susceptibility to lung cancer and to explore the sensitive genotypes of lung cancer so as to provide a basis for the screening of susceptible lung cancer patients and the early diagnosis of lung cancer patients and to make secondary preventive measures for lung cancer. Methods A molecular epidemiological study combining case-control study with restriction fragment length polymorphism in vitro amplification was used to detect the distribution of GSTP1 genotype. The relationship between GSTP1 gene polymorphism and susceptibility to lung cancer was analyzed. To evaluate the effect of GSTP1 mutation genotype on the incidence of lung cancer. Results There were 32 6 cases of GSTP1 gene polymorphism, of which 15 8 cases and 16 8 cases in the control group. Overall there was no statistically significant difference in the frequency of AA, AG and GG genotype distribution between case group and control group. In non-smoking men over 50 years old, those with GSTP1 mutation genotype (AG or GG) There was a significant statistical difference in the frequency of distribution between the control group and GSTP1 genotypes, and there was no interaction between GSTP1 mutation genotype and smoking in lung cancer. CONCLUSIONS: Men over 50 years of age who have a GSTP1 mutant genotype have a 13-fold greater risk of developing lung cancer than non-GSTP1-mutant genotypes