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目的:探讨中药复方益糖康对STZ大鼠蛋白激酶C(PKC)蛋白表达的影响。方法:采用STZ腹腔注射方法复制糖尿病大鼠模型,随机分为空白对照组、安慰剂组和益糖康组,灌胃6周后,采集大鼠背根神经节(DRG)标本,用免疫组化方法检测其PKC蛋白表达水平。结果:安慰剂组大鼠DRG中PKC蛋白表达水平显著上调,益糖康组较安慰剂组PKC蛋白表达水平显著降低。结论:中药复方益糖康可能抑制PKC激活,是其治疗糖尿病及其并发症的机制之一。
Objective: To investigate the effect of Yi Tang Kang on protein kinase C (PKC) protein expression in STZ rats. Methods: The model of diabetic rats was established by intraperitoneal injection of STZ. The rats were randomly divided into blank control group, placebo group and Yikangkang group. After 6 weeks of intragastric administration, DRG specimens of rats were collected, Method to detect PKC protein expression level. Results: The protein expression of PKC in DRG of placebo group was significantly up-regulated, and PKC protein expression in the Yixikang group was significantly lower than that in placebo group. Conclusion: Yikangkang, a traditional Chinese medicine compound, may inhibit the activation of PKC, which is one of its mechanisms in the treatment of diabetes mellitus and its complications.