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哺乳动物卵巢有大量的腔前卵泡,其中大部分卵泡不能进行排卵,而卵泡发育和闭锁的调节是一个高度复杂的过程。随着对卵泡生长过程的深入研究,国内外已经建立了多种体外培养方法,而腔前卵泡体外培养已成为现今的研究热点,并将其应用于雌性生殖毒理评价研究当中,为新药评价研究提供了可靠的实验工具。对哺乳动物腔前卵泡的体外培养中的胚胎来源、分离方法、培养条件和添加成分等几个方面进行了阐述,为进一步完善腔前卵泡培养体系提供实验依据。
Mammalian ovaries have a large number of preantral follicles, most of which follicles can not ovulation, and the regulation of follicular development and atresia is a highly complex process. With the in-depth study on the process of follicle growth, a variety of in vitro culture methods have been established both at home and abroad. However, the pre-implantation follicle culture in vitro has become a hot research topic nowadays and has been applied in the evaluation of female reproductive toxicity, Research provides a reliable experimental tool. The origin, methods of isolation, culture conditions and components of mammalian preantral follicles in vitro culture are described in several aspects, which provide experimental evidence for further improvement of pre-ovarian follicle culture system.