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基于有丝分裂素〔植物血凝素(PHA)、刀豆素A(ConA)、美洲商陆(PWM)〕刺激淋巴细胞,同时形成玫瑰花结以检出E受体和C_3受体,作者提出了一种同时作淋巴细胞亚群计数和功能测定的简易法。ConA和PWM能活化淋巴细胞C:受体的亲和力,增加酵母多糖-补体颗粒玫瑰花结形成细胞数(B细胞),并可形成既有酵母多糖-补体颗粒又有绵羊红细胞(SRBC)的混合玫瑰花结细胞(D细胞)。PHA能活化E受体,增加T细胞检出数。材料和方法取正常成人20例和患者33例(哮喘10例、湿疹15例和铍尘肺8例)的肝素抗凝血,经
Stimulating lymphocytes based on mitogens [PHA, ConA, Pheuolaeus alba] and rosettes to detect E receptors and C_3 receptors, the authors propose A Simple Method for Simultaneous Determination of Lymphocyte Subsets and Functions. ConA and PWM activate lymphocyte C: receptor affinity and increase the number of zymosan-complement granule rosette forming cells (B cells) and form a mixture of both zymosan-complement granule and sheep erythrocytes (SRBC) Rosette cells (D cells). PHA can activate E receptor, increase the number of T cells detected. Materials and Methods Heparin anticoagulant blood was obtained from 20 normal adults and 33 patients (10 with asthma, 15 with eczema and 8 with beryllium pneumoconiosis)