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用双水相萃取（ＡＴＰＥ）丙酰螺旋霉素（ＰＲＯ－ＳＰＭ），分别用ＰＲＯ－ＳＰＭ溶液和发酵液，对聚乙二醇（ＰＥＧ）和磷酸盐成相系统进行了研究，选出了合适的成相系统。在研究中发现ＰＲＯ－ＳＰＭ对成相有一定的影响。根据数据提出在ＰＲＯ－ＳＰＭ的双水相萃取过程中疏水作用是影响萃取的主要因素，与蛋白质以静电效应为主不同。用发酵液进行放大试验并与溶媒法进行比较，ＡＴＰＥ法萃取收率提高了３２％，而纯度有所下降。初步探讨了聚合物的重复使用。
The system of PEG and phosphate phase was studied by using PRO-SPM with PRO-SPM solution and fermentation broth, respectively, with the aid of aqueous two-phase extraction (ATPE) The right phase system. PRO-SPM was found to have a certain impact on the study in the study. According to the data, hydrophobicity is the main factor that affects the extraction in the aqueous two-phase extraction of PRO-SPM, which is different from the main effect of static electricity. With the fermentation broth amplification test and compared with the solvent method, ATPE extraction yield increased by 32%, while the purity decreased. Preliminary discussion of the polymer reuse.