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目的:鉴定花斑糠疹患者马拉色菌属的菌种构成,分析不同临床表现皮损的菌种分布情况。方法:刮取皮损处标本接种于改良的Leeming和Notman培养基。菌株通过生化方法鉴定。使用临床实验室标准研究所M27-A3方案对马拉色菌菌株进行体外药敏试验。结果:收集的200个样本中,73%来自男性,26%来自女性,好发年龄为21至30岁。最多见菌种为合轴马拉色菌(50%),继而是糠秕马拉色菌(32.86)、球形马拉色菌(14.28%)和斯洛菲马拉色菌(2.86%)。色素减退性皮损占74%,色素增加性皮损占26%。合轴与糠秕马拉色菌为色素减退性皮损最常见病原菌,球形和斯洛菲马拉色菌为色素沉着性皮损最常见病原菌。大多数菌种对抗真菌药物敏感。结论:合轴马拉色菌是引起花斑糠疹最常见病原菌。色素减退和色素沉着性花斑糠疹的菌种分布存在差异。改良的Leeming和Notman培养基可以用于马拉色菌的抗真菌药敏试验。
Objective: To identify the species composition of Malassezia in patients with pityriasis pityriasise, and to analyze the distribution of bacteria in different clinical manifestations. Methods: Skin lesions were scraped and inoculated into modified Leeming and Notman medium. Strains were identified by biochemical methods. In vitro susceptibility testing of Malassezia strains was performed using the Clinical Laboratory Standards Institute M27-A3 protocol. Results: Of the 200 samples collected, 73% were from males, 26% from females, and the prevalence was 21 to 30 years. The most common species was Malassezia occludens (50%), followed by Malassezia furfur (32.86), Malassezia globosa (14.28%) and P. sophori (2.86%). Pigment loss skin lesions accounted for 74%, pigmented lesions accounted for 26%. Co-shaft and chaff 秕 Malassezia hypogastric lesions is the most common pathogenic bacteria, spherical and Sopra pipiens malarial pigmented lesions are the most common pathogens. Most strains are antifungal drug sensitive. Conclusion: Malassezia is the most common pathogen causing pityriasis versicolor. There are differences in the distribution of pigmentation and pigmentation pityriasis versicolor. Modified Leeming and Notman media can be used for antifungal susceptibility testing of Malassezia.