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目的探讨白细胞介素10(IL-10)基因启动子-1082A/G、-819T/C多态性与中国早发冠心病的相关性。方法早发冠心病(病例组)92例,对照组94例,采用苯酚-氯仿法提取DNA,聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)分析IL-10基因启动子-1082A/G、-819T/C多态性。结果病例组与对照组IL-10-1082A/G,AA、AG、GG基因型频率及A、G等位基因频率差异均无统计学意义(均P>0·05);IL-10-819T/C,TT、TC、CC基因型频率及T、C等位基因频率差异亦均无统计学意义(均P>0·05)。结论IL-10基因启动子-1082A/G和-819T/C多态性可能与中国早发冠心病的易感性无关。
Objective To investigate the association of -1082A / G and -819T / C polymorphisms of interleukin 10 (IL-10) gene with premature coronary heart disease in China. Methods Ninety-two cases of premature coronary heart disease (case group) and 94 cases of control group were recruited. The DNA was extracted by phenol-chloroform method and analyzed by PCR-RFLP -1082A / G, -819T / C polymorphism. Results There were no significant differences in the frequencies of IL-10-1082A / G, AA, AG, GG genotypes and the frequencies of A and G alleles between the case group and the control group (all P> 0.05); IL-10-819T / C, TT, TC, CC genotype frequencies and T, C allele frequencies were also not statistically different (all P> 0.05). Conclusions The polymorphisms of IL-10 gene promoter -1082A / G and -819T / C may not be related to the susceptibility to premature coronary heart disease in China.