脱氧核酶是利用体外分子进化技术合成的一种具有催化功能的单链DNA片段 ,具有高效的催化活性和结构识别能力。迄今为止 ,已发现大量具有催化功能的脱氧核酶。其中具有RNA切割活性的脱氧核酶 ,能催化RNA特定部位的切割反应 ,从mRNA水平对基因灭活 ,从而调控蛋白质的表达 ,可能成为治疗肿瘤、病毒感染性疾病以及其它相关疾病 ,基因功能研究 ,核酸突变分析等的新型工具。 DNAzyme is a kind of single-stranded DNA fragment with catalytic function synthesized by in vitro molecular evolution technology, which has high catalytic activity and structure recognition ability. To date, a large number of catalytic deoxyribozymes have been found. Among them, DNAzyme with RNA cleavage activity can catalyze the cleavage reaction of specific parts of RNA and inactivate the gene at the mRNA level to regulate the protein expression, which may be used to treat tumor, viral infectious diseases and other related diseases. , Nucleic acid mutation analysis and other new tools.