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目的:探讨甲醛吸入急性肺水肿模型大鼠出、凝血时间的变化及地塞米松的干预作用,为其病理生理机制及治疗提供实验依据。方法:大鼠27只随机分为对照组、模型组和地米组,地米组于造模前0.5 h腹腔注射地塞米松1 mg/100 g体质量,其余两组注射等量生理盐水。按文献模型组和地米组以甲醛喷雾吸入制作急性肺水肿模型。染毒7 h后作出血时间和凝血时间测定,8 h后处死,取心、肺等器官称重并计算肺/心系数。对照组以生理盐水替代甲醛,处理方法同模型组。结果:与生理盐水对照组比较,模型组和地米组染毒后8 h体质量均显著降低(均P<0.01),肺湿重显著增大(均P<0.05),肺/心系数增大(P<0.05,P<0.01),出、凝血时间无显著性改变(均P>0.05)。结论:甲醛喷雾吸入造成的急性肺水肿大鼠出、凝血时间变化不明显,地塞米松干预无显著影响。
OBJECTIVE: To investigate the changes of clotting time and blood coagulation time in rats with acute pulmonary edema induced by formaldehyde inhalation, and the intervention effect of dexamethasone, providing the experimental evidence for its pathophysiological mechanism and treatment. Methods: Twenty-seven rats were randomly divided into control group, model group and dexamethasone group. The dexamethasone group was injected intraperitoneally with 0.5 mg / 100 g dexamethasone 0.5 h before modeling, and the other two groups were injected with the same amount of saline. Acute pulmonary edema model was induced by formaldehyde inhalation according to the literature model group and the dexamethasone group. 7 h after exposure to make blood time and coagulation time determination, 8 h after sacrifice, coring, lung and other organs were weighed and calculated lung / heart factor. The control group was replaced by normal saline, and the treatment method was the same as that of the model group. Results: Compared with the saline control group, the body weight of the model group and the dexamethasone group decreased significantly (all P <0.01) and lung wet weight (all P <0.05) (P <0.05, P <0.01), and there was no significant change in clotting time (all P> 0.05). Conclusion: There is no obvious change of clotting time in acute pulmonary edema rats induced by formaldehyde spray inhalation, and no significant effect of dexamethasone intervention.