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[目的]研究山药水提物(Aqueousextract of Dioscorea batatas,DA)对四氯化碳诱导肝损伤的肝功能的保护作用。[方法]建立CCl4诱导肝损伤的小鼠模型,测定正常对照组、造模组、阳性对照组、山药水提物100mg/kg、200mg/kg、400mg/kg、800mg/kg共7个组的血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)和肝组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)及组织蛋白含量。肝组织常规HE染色观察。[结果]与模型组比较,经山药水提物干预后,血清ALT、AST活性和肝组织中MDA、TNF-α、IL-1β含量明显降低(P﹤0.05),肝组织中SOD活性升高(P﹤0.05),组织蛋白含量差异无统计学意义(P﹥0.05)。[结论]山药水提物对四氯化碳造成的化学性肝损伤具有保护作用,并呈现一定的剂量依赖关系。
[Objective] To study the protective effect of Aqueousextract of Dioscorea batatas (DA) on hepatic function induced by carbon tetrachloride in liver injury. [Method] A mouse model of CCl4-induced liver injury was established. The normal control group, model group, positive control group, yam extract 100mg / kg, 200mg / kg, 400mg / kg and 800mg / kg Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver tissue superoxide dismutase (SOD), malondialdehyde (MDA), tumor necrosis factor alpha (TNF- -1β) and tissue protein content. Liver tissue routine HE staining. [Results] Compared with the model group, the activities of ALT and AST and the contents of MDA, TNF-α and IL-1β in liver tissue were significantly decreased (P <0.05) (P <0.05), while there was no significant difference in the content of tissue protein (P> 0.05). [Conclusion] The aqueous extract of Chinese yam has a protective effect on chemical liver injury caused by carbon tetrachloride, and shows a certain dose-dependent relationship.