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以50条彭亨丝虫3期幼虫腹股沟皮下接种感染无胸腺C_3H/HeN小鼠(裸鼠),取眶窦静脉血20mm~3计数微丝蚴,按Ash等法检获成虫。将海群生溶于pH7.2 PBS,每只鼠给予100mg/kg体重一次顿服治疗,对照组仅服同量的PBS,用于体外培养研究的海群生浓度为50mg/ml(PBS-BSA稀释)。从出生24小时内的杂交鼠摘取胸腺,置于含100U/ml青霉素和100mg/ml链霉素的
Athymic C_3H / HeN mice (nude mice) were inoculated subcutaneously with 50 Paenibacillus infestans into the groin of the third larvae. Microfilars of 20mm ~ 3 were taken from the orbital venous sinus and seized into adults by Ash et al. The sea group was dissolved in pH 7.2 PBS, each mouse given 100mg / kg body weight once a day treatment, the control group served only with the same amount of PBS for in vitro culture studies of sea group concentration of 50mg / ml (diluted in PBS-BSA ). Thymus was harvested from crossbred mice within 24 hours of birth and placed in a solution containing 100 U / ml penicillin and 100 mg / ml streptomycin