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[目的]研究甲氨喋呤-聚乳酸-羟基乙酸共聚物(MTX-PLGA)共聚物纳米囊的体外抑制效应。[方法]采用复乳法-溶剂挥发法制备MTX-PLGA共聚物纳米囊;用噻唑蓝(MTT)比色法检测不同浓度及不同时间MTX-PLGA共聚物纳米囊体外对人成骨肉瘤细胞株的抑制效应,以及与游离MTX对人骨肉瘤细胞株的抑制率相比较,并计算药物的半数抑制率IC50和对肿瘤细胞MG63的生长抑制率。[结果]不同浓度组在相同时间点的肿瘤细胞生长抑制率随药物浓度的增加而升高,不同浓度组间抑瘤率差异明显(P<0.05);在游离MTX组中,第3~6 d MTX-PL-GA组有显著的差异性(P<0.05)。[结论]MTX-PLGA共聚物纳米囊可以作为MTX的有效缓释载体,实现化疗药物控释释放,延长化疗药物对肿瘤的有效作用时间。
[Objective] To study the inhibitory effect of methotrexate-polylactic acid-glycolic acid copolymer (MTX-PLGA) nanocapsules in vitro. [Method] MTX-PLGA copolymer nanocapsules were prepared by double emulsion method and solvent evaporation method. MTT assay was used to detect the effect of MTX-PLGA copolymer nanocapsules on human osteosarcoma cell lines , And compared with the inhibitory rate of free MTX on human osteosarcoma cell line, and calculated the half-inhibitory rate of drug IC50 and the growth inhibition rate of tumor cell MG63. [Results] The inhibitory rates of tumor cell growth at different time points increased with the increase of drug concentration in different concentration groups, and there was significant difference (P <0.05) between different concentration groups. In the free MTX group, d MTX-PL-GA group was significantly different (P <0.05). [Conclusion] MTX-PLGA copolymer nanocapsules can be used as an effective sustained release carrier of MTX to achieve controlled release of chemotherapeutic drugs and prolong the effective time of chemotherapeutic drugs on tumors.