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目的观察松生拟层孔菌子实体乙醇提取物体外抗肿瘤活性。方法体外药效试验采用Alamar Blue法及MTT法,观察子实体乙醇提取物对人肺癌细胞株NCI-H460、小鼠肝癌细胞株H22及小鼠肉瘤细胞株S180的增殖抑制作用。结果其子实体乙醇提取物乙酸乙酯相在100μg/mL时,体外对NCI-H460、S180及H22细胞的增殖均有显著的抑制作用,其抑制率分别为98.73%、32.50%5、1.10%。结论松生拟层孔菌子实体乙醇提取物具有一定的体外抗肿瘤活性。
Objective To observe the antitumor activity of ethanol extract of Pinus ponderosa fruiting bodies in vitro. Methods In vitro efficacy test Alamar Blue method and MTT method to observe the fruiting body ethanol extract on human lung cancer cell line NCI-H460, mouse hepatoma cell line H22 and mouse sarcoma cell line S180 proliferation inhibition. Results The ethanol extract of its fruiting body ethyl acetate phase at 100μg / mL in vitro NCI-H460, S180 and H22 cell proliferation were significantly inhibited, the inhibition rates were 98.73%, 32.50% 5,1.10% . Conclusion The ethanol extract of Pinus pinaster has some anti-tumor activity in vitro.