用细胞贴附式和内面向外式膜片钳记录法研究豚鼠耳蜗单离外毛细胞底侧膜的钾离子单通道电流，记录到大电导型钙激活钾通道。在等修１４０ｍｍｏｌ／ＬＫＣｌ溶液时其电导为１６１±２６ｐｓ（内面向外式）或１５５±２７ｐｓ（细胞贴附式），反转电位为ｏｍＶ；浴液为Ｈａｎｋｓ液而电极向液为１４０ｍｍｏｌ／ＬＫＣＩ液时，电导为１３３±９ＰＳ（细胞贴附式）。当膜内面浴无钙液时通道活动消失。通道呈簇状发放或快速痪状发放方式，其开启和关闭时程直方图均可用三阶指数方程拟合。 Cell attachment and inward-outward patch-clamp recordings were used to investigate the single-channel potassium current in the basolateral membrane of the guinea pig cochlear isolated outer hair cells, and the large conductance calcium-activated potassium channels were recorded. The conductivity was 161 ± 26ps (inside-out) or 155 ± 27ps (cell-attached) when the solution was prepared with 140mmol / L KCl and the reversing potential was omV. The bath solution was Hanks solution and the electrode solution was 140mmol / L KCl When liquid, the conductance is 133 ± 9PS (cell attachment type). When the membrane inside the bath without calcium solution disappeared. Passages were distributed in clusters or rapid paralysis distribution method, the opening and closing time histograms can be used third-order exponential equation fitting.