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采用浸叶法对云南弥渡田间小菜蛾种群进行了8种杀虫剂毒力测定,并基于抗药性监测结果,制定了对弥渡县小菜蛾轮换用药和水旱轮作相结合的抗药性治理策略。抗药性监测结果表明,2011年,弥渡菜区小菜蛾种群对丁醚脲、苏云金杆菌敏感,LC_(50)分别为54.64、0.28 mg/L,抗性倍数分别为2.55、1.07;对氯虫苯甲酰胺、溴虫腈中抗,LC_(50)分别为6.37、21.27 mg/L,抗性倍数分别为28.17、53.19;对茚虫威、多杀菌素、高效氯氰菊酯和阿维菌素高抗,LC_(50)分别为69.03、13.75、1 114.41 mg/L和24.50 mg/L,抗性倍数分别为132.74、114.55、313.92和1 224.9。通过抗药性治理,到2014年弥渡菜区小菜蛾对氯虫苯甲酰胺、茚虫威和阿维菌素的抗性倍数明显下降,对氯虫苯甲酰胺由2011年的中抗恢复至敏感水平,对茚虫威由高抗下降至接近低抗水平,对阿维菌素的抗性倍数由1 224.9下降到652.75,对丁醚脲和苏云金杆菌处于低抗水平;对溴虫腈的抗性水平年度间变化不大,抗性倍数31.05~53.49,一直处于中抗水平。
Eight species of insecticides were tested for their toxicity to Plutella xylostella populations in Yunnan Province by dipping leaves method. Based on the results of drug resistance monitoring, drug resistance was developed for the combination of rotation and rotation of Plutella xylostella in Middu County Strategy. The resistance monitoring results showed that the population of Plutella xylostella was sensitive to diafenthiuron and Bacillus thuringiensis in 2011, the LC 50 values were 54.64 and 0.28 mg / L respectively, and the resistance multiples were 2.55 and 1.07 respectively. Benzamidine and chlorfenapyr, LC_ (50) were 6.37 and 21.27 mg / L, respectively. The resistance multiples were 28.17 and 53.19, respectively. For indoxacarb, spinosad, beta-cypermethrin and abamectin , LC 50 (50) were 69.03, 13.75, 114.41 mg / L and 24.50 mg / L, respectively. The resistance multiples were 132.74, 114.55, 313.92 and 1 224.9 respectively. By resistance management, the resistance to Plutella xylostella, Indoxacarb and Abamectin in Plutella xylostella decreased significantly in 2014, and the resistance to chlorantraniliprole in 2011 was restored to Sensitivity levels of indoxacarb decreased from high to near-low level of resistance, the avermectin resistance fold decreased from 1 224.9 to 652.75, diafenthiuron and Bacillus thuringiensis in a low anti-level; against chlorfenapyr The resistance level did not change much during the year, and the resistance ratio was 31.05 ~ 53.49, always at the level of resistance.