目的:建立草豆蔻药材的定性鉴别和定量检测方法。方法:运用薄层色谱法和高效液相色谱法对草豆蔻药材中山姜素(alpinetin)、乔松素(pinocembrin)、小豆蔻明(cardamonin)和桤木酮(alnustone)进行定性鉴别和含量测定。以UltimateXB-C18色谱柱(4.6 mm×250 mm,5μm)为固定相,甲醇-水为流动相,梯度洗脱,检测波长300 nm,柱温30℃。结果:草豆蔻药材中山姜素、乔松素、小豆蔻明和桤木酮的薄层色谱鉴别特征明显,专属性强;4个指标成分在进样量25.5~509(r=0.9999),24.9~498(r=0.999 9),26.1~521(r=0.999 9),50.2~1003 ng(r=0.999 9)与峰面积线性关系良好;平均加样回收率(n=9)分别为97.95%,97.36%,97.50%,98.02%,RSD均小于1.9%。结论:通过对9批不同来源的草豆蔻药材的薄层鉴别和含量测定,证明该方法简单灵敏,专属性强,准确可靠,重复性好,为全面评价草豆蔻药材质量提供了参考。 Objective: To establish a qualitative identification and quantitative detection of Herba Kadsura medicinal materials. Methods: The contents of alpinetin, pinocembrin, cardamonin and alnone were determined and identified by TLC and HPLC. Using the column of UltimateXB-C18 (4.6 mm × 250 mm, 5 μm) as stationary phase, methanol-water as mobile phase and gradient elution. The detection wavelength was 300 nm and the column temperature was 30 ℃. Results: The TLC characteristics of middle cassia root, johnsonin, cardamomine and almond ketone were distinct and specific. The four index components were in the range of 25.5 ~ 509 (r = 0.9999), 24.9 ~ 498 (r = 0.999 9), 26.1-521 (r = 0.999 9), 50.2-1003 ng (r = 0.999 9) showed a good linear relationship with the peak area. The average recoveries of n = 9 were 97.95% and 97.36% , 97.50% and 98.02%, respectively. The RSDs were less than 1.9%. Conclusion: The TLC identification and content determination of nine herbs from different origins prove that the method is simple, sensitive, accurate, reliable and reproducible. It provides a reference for the comprehensive evaluation of the quality of Medicinal Herbal Medicine.