Glycerol,dimethyl suffixod (DMSO) and acetamide were adopted to verify the cryoprotective ability of rnpenetrating cryoprotectants with three kinds of extenders for rabbit sperm. Three extenders (A,B and C) and rnthree level of the final concentrations of glycerol and DMSO (2% ,3% and 4% ),and acetamide 2% ,4% and rn5% were used with Extender A,4% glycerol had better cryoprotective effect for rabbit sperm motility and rn5% acetamide was better with Extender C. 4% acetamide was better in being combined with Extender A for rnsperm acrosome integrity ratio,5 % acetamide was better with Extender B and 2 % acetamide was better with rnExtende C. With extender A,there was not difference among three penetrating cryoprotectants for plasma rnmembrane integrity. With Extender B,3% glycerol was better than 4% acetamide for plasma membrane inrntegrity ,and 3 % glycerol with Extender C was better than 2 0%－ 4 % DMSO and 2 % acetamide.