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介绍了氯金酸直接还原法制备胶体金的改进方法,通过本方法获得了分散性好、粒径在5-14nm的金纳米颗粒.结合表面活性剂的两亲性和纳米微粒本身的高比表面积引起的金粒子电荷表面分布变化,探讨了两亲分子有序组合体在纳米合成中的作用,研究表明SDS在纳米颗粒形成过程中先与Au3+形成复合体,同时在Au3+被还原为Au并聚集成纳米颗粒时SDS起到了稳定剂和分散剂的作用,阻止了纳米金的进一步团聚,使金粒子的粒径分散比较均匀,尺度可控.初步结果表明本方法制备的纳米金颗粒对蛋白质(如BSA等)有良好的吸附性能,并有可能发展简便快速的蛋白质测定方法.
An improved method for preparing colloidal gold by direct reduction of chloroauric acid is introduced, and gold nanoparticles with good dispersibility and particle size of 5-14 nm are obtained by the method. The amphiphilicity of the combined surfactant and the nanoparticle itself Surface area caused by changes in the charge distribution of gold particles to explore the orderly combination of amphiphiles in nanosynthesis in the role of research shows that SDS in the formation of nanoparticles with Au3 + form a complex while Au3 + is reduced to Au and When aggregated into nanoparticles, SDS played a role of stabilizer and dispersant, preventing the further agglomeration of gold nanoparticles, making the particle size of gold particles dispersed uniformly and controlling the scale.The preliminary results show that the gold nanoparticles prepared by the method have the advantages of inhibiting the protein (Such as BSA, etc.) have good adsorption properties, and it is possible to develop simple and rapid protein assay.