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目的:对江南卷柏不同部位提取物在体外抗单纯疱疹病毒Ⅰ型(HSV-Ⅰ)的作用进行研究,并对其抗病毒作用机制进行初步探讨。方法:采用不同部位的提取物培养HSV-I感染的Hep-2细胞,观察比较细胞病变效应(CPE),72h后以空斑减数法测定细胞活性。结果:在Hep-2细胞体系中,江南卷柏各不同部位提取物均具有一定的抑制HSV-Ⅰ对细胞的致病变作用,使细胞存活率提高,其中含有脂溶性双黄酮和水溶性黄酮苷的部分是主要的活性部位。结论:江南卷柏可以作为新潜在的抗HSV-Ⅰ的资源植物。
OBJECTIVE: To study the anti-herpes simplex virus type 1 (HSV-Ⅰ) in vitro extracted from different parts of southern Chinese cabbage and study its anti-viral mechanism. Methods: Hep-2 cells infected by HSV-1 were cultured with extracts of different parts. The cytopathic effect (CPE) was observed and compared. After 72 hours, the cell viability was measured by plaque reduction method. Results: In Hep-2 cell system, the extracts from different parts of the stem of Selaginella tamarisse had the same inhibitory effect on the pathogenicity of HSV-Ⅰ cells, which led to the increase of cell survival rate, which included fat-soluble biflavonoids and water-soluble flavonoids The glycoside moiety is the major active site. Conclusion: Selaginella polygamy can be used as a new potential plant resource for anti-HSV-Ⅰ.