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变性梯度凝胶电泳(Denatured Gradient GelElectrophoresis,DGGE)技术是一种能将大小相同而序列不同的DNA分子分离的分子生物学技术,无需经过传统培养方法,便能分析环境和人体微生态菌群的组成和结构,具有高通量、省时、经济等优点。该文从该技术在根管感染、龋病、牙周微生物、口腔生物膜等方面的应用进行综述。
Denatured Gradient Gel Electrophoresis (DGGE) is a molecular biology technique that separates DNA molecules with the same size and different sequences. It can analyze the environment and the microbial flora of the human body without traditional culturing methods Composition and structure, with high throughput, saving time, economic advantages. This article reviews the application of this technology in root canal infection, dental caries, periodontal microorganisms and oral biofilms.