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为探讨真核翻译延伸因子1A2在宫颈癌中的表达以及临床病理学意义。收集新鲜宫颈癌组织和宫颈正常组织样本各10例,运用实时荧光定量PCR方法检测了eEF1A2基因的mRNA表达水平。收集宫颈癌患者60例、宫颈上皮内瘤样病变患者45例和慢性宫颈炎患者35例石蜡标本组织,采用免疫组织化学SP方法检测eEF1A2基因在宫颈组织中的蛋白表达状况。qRT-PCR结果显示,eEF1A2基因在宫颈癌组织mRNA表达水平较正常宫颈组织明显增加,差异有统计学意义(P<0.01)。免疫组织化学结果显示,eEF1A2蛋白质表达强阳性率、弱阳性率和阴性率表达在宫颈癌组织分别为46.6%,31.0%和22.4%,而在慢性宫颈炎组织中分别为21.2%,48.5%和30.3%,差异具有统计学意义(P<0.001)。由此可知,eEF1A2基因的表达增高可能在宫颈癌的发生、发展中发挥一定的作用,其机制有待进一步研究,观察eEF1A2表达情况对于宫颈癌的临床诊断、病理分级有参考价值。
To investigate the expression of eukaryotic translation elongation factor 1A2 in cervical cancer and its clinicopathological significance. Ten samples of fresh cervical cancer tissues and normal cervical tissues were collected and the expression of eEF1A2 mRNA was detected by real-time fluorescence quantitative PCR. Sixty cases of cervical cancer, 45 cases of cervical intraepithelial neoplasia and 35 cases of chronic cervicitis were collected. The expression of eEF1A2 in cervical tissue was detected by immunohistochemical SP method. The results of qRT-PCR showed that the mRNA expression of eEF1A2 in cervical cancer tissues was significantly increased compared with normal cervical tissues (P <0.01). The results of immunohistochemistry showed that the expression rates of eEF1A2 protein were 46.6%, 31.0% and 22.4% in cervical cancer tissues, 21.2% and 48.5% in chronic cervicitis tissues respectively 30.3%, the difference was statistically significant (P <0.001). It can be seen, eEF1A2 gene expression may play an important role in the occurrence and development of cervical cancer, the mechanism remains to be further studied, observe the expression of eEF1A2 for cervical cancer clinical diagnosis, pathological grade with reference value.