【摘 要】
:
In 1969, Emil Wolf proposed diffraction tomography using coherent holographic imaging to extract 3D information from transparent, inhomogeneous objects. In the
【机 构】
:
Quantitative Light Imaging Laboratory,Beckman Institute for Advanced Science and Technology,Departme
论文部分内容阅读
In 1969, Emil Wolf proposed diffraction tomography using coherent holographic imaging to extract 3D information from transparent, inhomogeneous objects. In the same era, the Wolf equations were first used to describe the propagation correlations associated with partially coherent fields. Combining these two concepts, we present Wolf phase tomography (WPT), which is a method for performing diffraction tomography using partially coherent fields. WPT reconstruction works directly in the space–time domain, without the need for Fourier transformation, and decouples the refractive index (RI) distribution from the thickness of the sample. We demonstrate the WPT principle using the data acquired by a quantitative-phase-imaging method that upgrades an existing phase-contrast microscope by introducing controlled phase shifts between the incident and scattered fields. The illumination field in WPT is partially spatially coherent (emerging from a ring-shaped pupil function) and of low temporal coherence (white light), and as such, it is well suited for the Wolf equations. From three intensity measurements corresponding to different phase-contrast frames, the 3D RI distribution is obtained immediately by computing the Laplacian and second time derivative of the measured complex correlation function. We validate WPT with measurements of standard samples (microbeads), spermatozoa, and live neural cultures. The high throughput and simplicity of this method enables the study of 3D, dynamic events in living cells across the entire multiwell plate, with an RI sensitivity on the order of 10?5.
其他文献
Two-dimensional (2D) transition metal dichalcogenides (TMDCs) and graphene compose a new family of crystalline materials with atomic thicknesses and exotic mech
Inspired by the“run-and-tumble”behaviours of Escherichia coli (E. coli) cells, we develop opto-thermoelectricmicroswimmers. The microswimmers are based on die
The measurement of the optical transmission matrix (TM) of an opaque material is an advanced form of space-variant aberration correction. Beyond imaging, TM-bas
Mid-infrared (MIR) light-emitting devices play a key role in optical communications, thermal imaging, and material analysis applications. Two-dimensional (2D) m
Thouless charge pumping protocols provide a route for one-dimensional systems to realize topological transport. Here, using arrays of evanescently coupled optic
Using an array of coupled microwave resonators arranged in a deformed honeycomb lattice, we experimentally observe the formation of pseudo-Landau levels in the
Inspired by the revolutionary impact of super-resolution fluorescence microscopy, super-resolution Raman imaging has been long pursued because of its much highe
Multicolour single-mode polarized microlasers with visible to near-infrared output have very important applications in photonic integration and multimodal bioch
Advanced 1.5-μm emitting materials that can be used to fabricate electrically driven light-emitting devices have the potential for developing cost-effective li
Recently, integrated photonics has attracted considerable interest owing to its wide application in optical communication and quantum technologies. Among the nu