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目的:探讨何首乌、三七提取物预防大鼠非酒精性脂肪性肝炎及其肝组织PPARα、PGC–1α表达的影响。方法:32只雄性SD大鼠,随机分为4组,每组8只。除正常对照组外,高脂饲料喂养10周建立大鼠非酒精性脂肪性肝炎模型。于造模即日起,正常对照组、模型组每天分别给予10mL/kg饮用水,中药组给予何首乌、三七提取物,易善复组给予易善复灌胃。给药10周后,处死所有大鼠,检测血清LDL、HDL、TG,ALT、AST、GGT;肝组织TG和PPARα、PGC–1αmRNA水平。结果:中药组血清LDL含量较模型组下降(P<0.05);中药组血清HDL、TG,ALT、AST、GGT和肝组织TG含量较模型组显著下降(P<0.01),肝组织PPARα、PGC–1αmRNA水平较模型组显著增高(P<0.01)。结论:何首乌、三七提取物可降低NASH大鼠血清、肝脏脂质合成与聚积,减少炎症反应,从而有效防治NASH,具有良好的临床应用前景。
Objective: To investigate the effect of extracts of Polygonum multiflorum Thunb. And Radix Notoginseng on the prevention of nonalcoholic steatohepatitis in rats and the expression of PPARα and PGC-1α in liver tissue. Methods: Thirty-two male SD rats were randomly divided into 4 groups, 8 in each group. In addition to the normal control group, a non-alcoholic steatohepatitis model was established by feeding high-fat diet for 10 weeks. As of the date of model establishment, the normal control group and the model group were given 10 mL/kg of drinking water each day. The Chinese medicine group was given the extract of Polygonum multiflorum and Radix notoginseng. The Yishan Fu group was given Yishanfu gavage. Ten weeks after administration, all rats were sacrificed and serum LDL, HDL, TG, ALT, AST, and GGT were measured; mRNA levels of TG, PPARα, and PGC-1α in liver tissue were measured. Results: Serum LDL levels in Chinese Herbal Medicine group were lower than those in model group (P<0.05); Serum HDL, TG, ALT, AST, GGT and liver TG content in Chinese Herbal Medicine group were significantly lower than those in model group (P<0.01). Liver tissue PPARα, PGC The level of –1α mRNA was significantly higher than that of the model group (P<0.01). Conclusion: Extracts of Polygonum multiflorum and Radix notoginseng can reduce lipid synthesis and accumulation in serum and liver of NASH rats, reduce inflammation, and effectively prevent and treat NASH. It has good clinical application prospects.