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目的 探讨survivin基因在化疗药物顺铂 (DDP)和足叶乙苷 (VP16 )诱导的肺癌细胞凋亡中的作用。方法 用肺腺癌A5 4 9细胞株进行培养传代 ,MTT试验检测DDP和VP16不同药物浓度对肺癌细胞生长的抑制作用 ,实验分为 :DDP、VP16和对照组。DDP和VP16组的肺癌细胞又分为高浓度和低浓度化疗药物处理组。用逆转率PCR检测sur vivin基因的表达。同时利用流式细胞术定量检测细胞凋亡。结果 不同浓度VP16和DDP对肺癌细胞生长均有明显的抑制作用 ,并有浓度和时间的依赖性。化疗药物组细胞凋亡率和survivin基因表达抑制率高于对照组 ,也有时间依赖性。结论 survivin基因的抑制在DDP和VP16诱导肺癌细胞凋亡中起重要作用
Objective To investigate the role of survivin gene in the apoptosis of lung cancer cells induced by cisplatin (DDP) and etoposide (VP16). Methods The lung adenocarcinoma A549 cell line was cultured and passaged. MTT assay was used to detect the inhibitory effect of different concentrations of DDP and VP16 on the growth of lung cancer cells. The experiment was divided into two groups: DDP, VP16 and control group. The lung cancer cells in DDP and VP16 groups were further divided into high and low concentration chemotherapy drug treatment groups. Survivin gene expression was detected by reverse transcription PCR. At the same time, flow cytometry was used to quantitatively detect apoptosis. Results Different concentrations of VP16 and DDP significantly inhibited the growth of lung cancer cells in a concentration-dependent and time-dependent manner. Chemotherapy group apoptosis rate and survivin gene expression inhibition rate was higher than the control group, but also time-dependent. Conclusion The inhibition of survivin gene plays an important role in the apoptosis of lung cancer cells induced by DDP and VP16