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目的建立高岭土诱导大鼠交通性脑积水的模型。方法雄性Wistar大鼠40只,随机将其分为对照组和实验组。实验组28只大鼠应用显微外科技术在显微镜下向枕大池中注射2%高岭土混悬液0.05mL,对照组12只大鼠应用显微外科技术在显微镜下向枕大池中注射生理盐水0.05mL。分别在注射后第28天处死大鼠,做脑室病理切片分析,测定脑室体积大小,实验组与对照组进行对比,并确定脑积水是否形成。结果实验组有22只大鼠成功诱发脑积水,且脑室随着时间的延长而逐渐扩大。结论应用高岭土混悬液注射法可成功诱导大鼠交通性脑积水,适用于急、慢性交通性脑积水的实验研究。
Objective To establish a model of kaolinite induced traffic hydrocephalus in rats. Methods Forty male Wistar rats were randomly divided into control group and experimental group. 28 rats in the experimental group were injected with 0.05% 2% kaolin suspension into the occipital cistern by microsurgical technique under the microscope, and 12 rats in the control group were injected with physiological saline 0.05 mL. Rats were sacrificed on the 28th day after injection, respectively. Pathological analysis of the ventricles was performed to determine the ventricular volume. The experimental group and the control group were compared to determine whether hydrocephalus was formed. Results Twenty-two rats in the experimental group were successfully induced hydrocephalus, and the ventricles gradually enlarged with time. Conclusion Kaolin suspension injection can successfully induce traumatic hydrocephalus in rats and is suitable for the experimental study of acute and chronic hydrocephalus.