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背景:研究表明电磁场可调节骨髓间充质干细胞的增殖和分化,但其具体机制尚不清楚。目的:从ERK信号途径探讨电磁场诱导大鼠骨髓间充质干细胞增殖与分化成骨的作用。方法:取第3代生长良好的大鼠骨髓间充质干细胞,暴磁组给予15Hz、1mT的正弦波电磁场刺激,PD98059+暴磁组在电磁场刺激前给予20μmol/L ERK阻断剂PD98059,PD98059组仅给PD98059不进行电磁场刺激,对照组正常培养。电磁场刺激后,收集各组细胞,Western blot法检测ERK通路的活性,MTT法检测细胞增殖活性,碱性磷酸酶试剂盒检测细胞碱性磷酸酶活性。结果与结论:电磁场刺激后,细胞ERK1/2磷酸化水平、细胞的增殖活性、及碱性磷酸酶活性均明显升高(P<0.01);PD98059可明显抑制ERK1/2磷酸化水平及细胞增殖活性的升高(P<0.01),而在一定程度上提高细胞的碱性磷酸酶活性(P<0.01)。说明电磁场刺激可通过激活骨髓间充质干细胞ERK信号通路,并且主要通过该途径促进骨髓间充质干细胞的增殖;而在脉冲电磁场促进骨髓间充质干细胞分化成骨的过程中,激活ERK信号通路所起的作用较小。
Background: Studies have shown that electromagnetic fields can regulate the proliferation and differentiation of bone marrow-derived mesenchymal stem cells, but the exact mechanism is not clear. OBJECTIVE: To investigate the effect of electromagnetic field on the proliferation and differentiation of rat bone marrow mesenchymal stem cells from the ERK signaling pathway. METHODS: The third generation of rat bone marrow-derived mesenchymal stem cells grew well. The rats were exposed to sinusoidal electromagnetic waves of 15Hz and 1mT in the magnetic burst. PD98059 and PD98059 were given 20μmol / L ERK blockers before the electromagnetic field stimulation PD98059 only did not stimulate the electromagnetic field, the control group normal culture. After stimulated by electromagnetic field, the cells in each group were collected, the activity of ERK pathway was detected by Western blot, the cell proliferation activity was detected by MTT assay, and the alkaline phosphatase activity was detected by alkaline phosphatase kit. RESULTS AND CONCLUSION: The phosphorylation of ERK1 / 2, the activity of cell proliferation and the activity of alkaline phosphatase were significantly increased after electromagnetic field stimulation (P <0.01). PD98059 significantly inhibited the phosphorylation of ERK1 / 2 and cell proliferation (P <0.01), but increased the alkaline phosphatase activity (P <0.01) to a certain extent. It is concluded that EM stimulation can activate the ERK signaling pathway of BMSCs and promote the proliferation of BMSCs mainly through this pathway. However, in the process of pulsed electromagnetic fields promoting the differentiation of BMSCs into osteoblasts, ERK signaling pathway The role played by smaller.