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从北京通州地区芹菜软腐病样中分离获得的菌株QC01在实验室条件下能浸软芹菜叶柄组织,并能引起温室种植的芹菜软腐症状的产生。经病原菌表型特征分析,确定该菌株为有鞭毛的革兰氏阴性果胶杆菌Pectobacterium。QC01与参试的P.carotovorum subsp.odoriferum(Pco)菌株T5和CC1均具备在37℃以及在含有7%Na Cl培养基中生长的能力;能分解柠檬酸盐和液化明胶;除常见碳源外,QC01还能降解α-甲基葡糖苷、麦芽糖、山梨醇、阿拉伯糖和阿拉伯半乳聚糖。基于16S rRNA基因完整序列系统发育关系表明,QC01与其他Pco菌株聚集成明显的Pco类群。QC01和CC1 type II与已报道的Pco菌株JKI 582 type II和NB 1892的16S rRNA基因完整序列相似性为100%。这是首次在中国发现Pectobacterium carotovorum subsp.odoriferum引发芹菜软腐病。
Strain QC01 isolated from celery soft rot samples in Tongzhou, Beijing, can soak celery petiole tissue under laboratory conditions and cause the production of celery soft rot in greenhouse cultivation. The phenotypic characteristics of the pathogen analysis to determine the strain is flagellated Gram-negative Pectobacterium. Both QC01 and the tested P.carotovorum subsp. Odoriferum (Pco) strains T5 and CC1 have the ability to grow at 37 ° C and in medium containing 7% NaCl; decompose citrate and liquefy gelatin; besides common carbon sources In addition, QC01 degrades α-methylglucoside, maltose, sorbitol, arabinose and arabinogalactan. Phylogenetic relationships based on the complete sequence of the 16S rRNA gene showed that QC01 and other Pco strains aggregated into distinct Pco-like groups. The complete sequence similarity of QC01 and CC1 type II with the 16S rRNA gene of reported Pco strains JKI 582 type II and NB 1892 was 100%. This is the first time in China that Pectobacterium carotovorum subsp. Odoriferum was found to cause soft rot in celery.