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目的 :研究调肝导浊中药诱导主动脉平滑肌细胞一氧化氮及抑制细胞增殖的作用机制。方法 :运用体外培养技术 ,培养大鼠主动脉SMC ,以加高脂血清和加中药等不同培养基进行分组培养 ,取细胞及培养液进行指标检测。结果 :(1)调肝导浊中药可明显抑制高脂血清培养下SMC的增殖 ,其PCNA阳性率低于造模组。(2)调肝导浊中药可增强体外培养SMCiNOS表达 ,增加细胞培养液NO含量。结论 :该法中药可对SMC增殖起到良好调控作用 ,从而阻止AS发生发展
Objective : To study the mechanism of the induction of nitric oxide in aortic smooth muscle cells and the inhibition of cell proliferation by Tiaoganzhuzhu herbs. Methods : In vitro culture techniques were used to culture rat aorta SMCs, and cultured in groups of different media such as hyperlipidemia and Chinese medicine. Cells and culture fluids were used for index detection. Results : (1) The liver-regulating and Turbidity-removing Chinese herbs can obviously inhibit the proliferation of SMC under hyperlipidemic serum culture. The positive rate of PCNA is lower than that of the model-building group. (2) Liver-regulating and muddy Chinese herbs can enhance the expression of SMciNOS in vitro and increase the NO content in cell culture fluid. Conclusion : This method of Chinese medicine can play a good role in the regulation of SMC proliferation, thus preventing the development of AS