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目的 :构建两株IL 15末端缺失突变体以研究其N、C端对其功能的影响。方法 :以pBV2 2 0为载体 ,在大肠杆菌中进行表达。表达产物以包涵体形式存在 ,经过SDS PAGE纯化 ,复性 ,以CTLL 2增殖实验检测野生型及突变体的生物活性。结果 :N端缺失突变体失去了增殖活性 ,而C端缺失突变体保持了对CTLL 2的生长刺激活性
OBJECTIVE: To construct two IL15 deletion mutants to study the effect of N and C termini on their function. Methods: pBV220 was used as a vector and expressed in E. coli. The expressed product was in the form of inclusion bodies, purified by SDS PAGE and renatured. The biological activity of wild type and mutant was detected by CTLL 2 proliferation assay. Results: The N-terminal deletion mutant lost its proliferative activity whereas the C-terminal deletion mutant retained its growth stimulating activity on CTLL2