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以２，９－二甲基－１，１０－菲咯啉为初始原料，合成了２，９－二甲基－１，１０－菲咯啉的ｄｌ－丙氨酸衍生物：１，１０－菲咯啉－２，９－二亚甲基亚氨基－（２，２’－二甲基）二乙酸（Ｌ）。该配体经过元素分析。红外光谱、核磁共振氢谱表征。在 ２５±０．１℃、 Ｉ＝ ０．１ｍｏｌ·ｄｍ~（－３）ＮａＮＯ_３的条件下，用ｐＨ电位滴定法测定了该配体的质子化常数及其与Ｌａ（Ⅲ）的配合物的稳定常数。通过电子光谱研究了Ｌｂ（Ⅲ）的配合物与小牛胸腺 ＤＮＡ的相互作用。用溴化乙锭作为荧光探针研究了 Ｌａ（Ⅲ）－Ｌ配合物与小牛胸腺 ＤＮＡ的相互作用的过程。作为对比，也研究了 Ｌａ（Ⅲ）分别与１，１０－菲咯啉（Ｐｈｅｎ）、 ｄｌ－丙氨酸（ Ａｌａ）的配合物与小牛胸腺 ＤＮＡ的相互作用。结果表明 Ｌａ（Ⅲ）－Ｌ配合物与小牛胸腺ＤＮＡ作用既有共价键合，又有插入作用。
Starting from 2,9-dimethyl-1,10-phenanthroline, dl-alanine derivatives of 2,9-dimethyl-1,10-phenanthroline were synthesized: 1,10- Phenanthroline-2,9-dimethyleneimino- (2,2’-dimethyl) diacetic acid (L). The ligand undergoes elemental analysis. IR, 1H NMR characterization. Under the conditions of 25 ± 0.1 ℃ and I = 0.1mol · dm -3 NaNO_3, the protonation constants of the ligands and their complexes with La (Ⅲ) were determined by pH potentiometric titration Stability constant. The interaction between Lb (Ⅲ) complexes and calf thymus DNA was studied by electron spectroscopy. The procedure of interaction between La (Ⅲ) -L complex and calf thymus DNA was investigated using ethidium bromide as a fluorescent probe. In contrast, the interaction between La (III) and 1,10-phenanthroline (Phen) and dl-alanine (Ala) complexes with calf thymus DNA was also investigated. The results showed that the La (III) -L complex interacted with calf thymus DNA both covalently and inserted.