目的　探讨抗原特异性无能T淋巴细胞的诱导及其耐受特性。方法　在体外建立抗原递呈细胞 T淋巴细胞 B淋巴细胞反应系统 ,利用环孢素A(CsA)与抗B7 1单克隆抗体联用阻断B7:CD2 8共刺激途径 ,采用3H TdR法检测T淋巴细胞增殖 ,酶联免疫吸附试验 (ELISA法 )测定B淋巴细胞分泌的抗体。结果　CsA与抗B7 1单克隆抗体联用可诱致T淋巴细胞无能 ,无能T淋巴细胞不产生白细胞介素 2 (IL 2 ) ;外源性IL 2能阻止T淋巴细胞无能的诱导 ,但不能逆转这一耐受状态。结论　CsA与抗B7 1单克隆抗体联用可以诱导T淋巴细胞的抗原特异性耐受。 Objective To investigate the induction of antigen-specific incompetent T lymphocytes and their tolerance characteristics. Methods The B lymphocyte reaction system of antigen presenting cells (T lymphocytes) was established in vitro. The co-stimulatory pathway of B7: CD28 was blocked by cyclosporin A (CsA) and anti-B7 1 monoclonal antibody. The 3H TdR method was used to detect T Lymphocyte proliferation and enzyme-linked immunosorbent assay (ELISA) were used to measure the antibodies secreted by B lymphocytes. Results CsA combined with anti-B7 1 monoclonal antibody could induce the inability of T lymphocytes to produce interleukin 2 (IL 2); exogenous IL 2 prevented the induction of T lymphocyte impotence but failed to reverse This state of tolerance. Conclusion The combination of CsA and anti-B7 1 monoclonal antibody can induce the antigen-specific tolerance of T lymphocytes.