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目的探讨载体介导的转化生长因子(TGF)-β1小干扰RNA(siRNA)对人胃癌细胞株SGC-7901生物学行为的影响。方法构建TGF-β1特异性siRNA真核表达载体。转染胃癌细胞72 h后,以半定量逆转录一聚合酶链反应(RT-PCR)、Western blot检测细胞中TGF-β1的mRNA和蛋白表达的变化；以噻唑蓝(MTT)比色法、流式细胞术、细胞附壁试验和Miliicell小室测定细胞增殖、周期、体外黏附和侵袭力,并观察裸鼠体内成瘤性。结果与未处理组比较,TGF-β1-siRNA载体能明显下调TGF-β1 mRNA[(0．910±0．066)与(0．617±0．025)比较,P＜0．01]和蛋白[(120．00±8．72)与(41．00±5．57)比较,P＜0．01]。在胃癌细胞中的表达；细胞抑制率达30．18%,体外黏附、侵袭力下降,裸鼠体内成瘤性受到抑制。结论载体介导的TGF-β1 siRNA可显著下调TGF-β1在胃癌细胞中的表达,在一定程度上抑制其恶性生物学行为。
Objective To investigate the effect of carrier-mediated transforming growth factor-β1 (siRNA) on the biological behavior of human gastric cancer cell line SGC-7901. Methods The eukaryotic expression vector of TGF-β1 siRNA was constructed. The expression of TGF-β1 mRNA and protein was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blot 72 h after transfected into gastric cancer cells. MTT assay, Flow cytometry, cell wall adhesion assay and Miliicell chamber were used to determine cell proliferation, cell cycle, in vitro adhesion and invasiveness, and tumorigenicity in nude mice was observed. Results Compared with the untreated group, the TGF-β1-siRNA vector significantly downregulated the expression of TGF-β1 mRNA [(0.910 ± 0.066) vs. (0.617 ± 0.025), P <0.01] [(120.00 ± 8.72) vs (41.00 ± 5.57), P <0.01]. In gastric cancer cells, the cell inhibition rate reached 30.18%, adhesion in vitro and invasiveness decreased, and the tumorigenicity in nude mice was inhibited. Conclusion The carrier-mediated TGF-β1 siRNA can down-regulate the expression of TGF-β1 in gastric cancer cells to a certain extent, inhibit the malignant biological behavior.