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我们用平皿测活法研究了江浙蝮蛇毒对真菌和大肠杆菌生长的抑制作用,并对其抗细菌的组分进行柱层析分离纯化,得到组分单一的LAO:不仅有抑菌作用还有L-氨基酸氧化酶的活性。纯化后LAO的比活力为808U/mg。蛇毒经酸性聚丙烯酰胺电泳后测抑菌活性发现有3个抑菌组分(Ⅰ、Ⅱ、Ⅲ),LAO为抑菌组分Ⅱ。
We studied the inhibitory effect of Agkistrodon acutus, Jiangsu and Zhejiang on the growth of fungi and Escherichia coli by plate test, and separated and purified the antibacterial components by column chromatography to get a single component of LAO: not only antibacterial effect but also L-amino acid oxidase activity. The specific activity of purified LAO was 808U / mg. The results showed that there were three antibacterial components (Ⅰ, Ⅱ and Ⅲ) in the venom polyacrylamide electrophoresis and the antibacterial component Ⅱ.