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目的:观察红豆杉多糖对心肌缺血再灌注损伤(MIRI)导致的心肌细胞凋亡的影响,并初步探讨其可能机制。方法:将30只比格犬随机分为假手术组、模型组、红豆杉多糖低、高剂量组、卡维地洛对照组,每组6只,分别给药7d后,建立MIRI模型。采用TUNEL染色法标记缺血再灌注区凋亡心肌细胞,计数凋亡指数(AI);采用RT-PCR法及Western印迹法检测缺血再灌注区心肌Fas mRNA及蛋白表达。结果:模型组、低剂量组心肌细胞AI较假手术组显著增高(P<0.01);高剂量组、卡维地洛组心肌细胞AI显著低于模型组和低剂量组(P<0.05),与假手术组比较无显著差异;模型组及低剂量组Fas mRNA及蛋白表达较假手术组显著升高(P<0.01);各治疗组Fas mRNA及蛋白表达较模型组显著降低(P<0.01),且高剂量组、卡维地洛组Fas mRNA及蛋白表达显著低于低剂量组(P<0.05,P<0.01),与假手术组比较无显著差异。结论:抑制Fas mRNA及蛋白表达可能是红豆杉多糖抗缺血再灌注导致的心肌细胞凋亡的机制之一。
Objective: To observe the effect of Taxus polysaccharides on cardiomyocyte apoptosis induced by myocardial ischemia-reperfusion injury (MIRI) and to explore its possible mechanism. Methods: Thirty Beagle dogs were randomly divided into sham operation group, model group, polysaccharide low dose group, high dose group and carvedilol control group, with 6 rats in each group. MIRI models were established after administration for 7 days. The apoptotic cardiomyocytes were labeled with TUNEL staining and the apoptotic index (AI) was counted. The expression of Fas mRNA and protein in myocardium of ischemia-reperfusion area was detected by RT-PCR and Western blot. Results: Compared with sham operation group, AI in model group and low dose group was significantly higher than that in sham operation group (P <0.01); AI in high dose group and carvedilol group was significantly lower than that in model group and low dose group (P <0.05) The expression of Fas mRNA and protein in model group and low dose group were significantly higher than those in sham operation group (P <0.01). The expression of Fas mRNA and protein in each group was significantly lower than that in model group (P <0.01) ), And the expressions of Fas mRNA and protein in high-dose and carvedilol groups were significantly lower than those in low-dose group (P <0.05, P <0.01). There was no significant difference between the two groups. Conclusion: Inhibition of Fas mRNA and protein expression may be one of the mechanisms by which Taxus polysaccharides can prevent cardiomyocyte apoptosis induced by ischemia-reperfusion.