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目的:探讨小白菊内酯(parthenolide,PN)对球囊损伤后内膜增殖的影响及其可能机制。方法:通过球囊损伤联合高脂喂养建立兔髂血管炎症反应及内膜增殖模型,将30只兔高脂适应性喂养1周后随机分为6组:对照组:假手术+0.9%生理盐水(normal saline,NS)(n=5);假手术+4%二甲基亚砜(dimethyl sulfoxide,DMSO)(n=5);模型组:球囊损伤+0.9%NS(n=5);球囊损伤+4%DMSO(n=5);治疗组(PN溶解于4%DMSO):低剂量:球囊损伤+PN 1 mg/(kg·d)(n=5);高剂量:球囊损伤+PN 2 mg/(kg·d)(n=5),假手术组分离结扎股动脉不行球囊损伤,术后立即按分组给药。4周后检测比较各组血管内膜增殖、核转录因子-κB(nuclear transcription factor-κB,NF-κB)、白介素(interleukin,IL)-6和肿瘤坏死因子(tumor necrosis factor,TNF)-α的表达。结果:模型组与对照组比较,内膜厚度、NF-κB、IL-6和TNF-α明显增加(P<0.05);治疗组与模型组比较,上述指标在高剂量组显著降低(P<0.05)。结论:PN通过抑制NF-κB及其下游炎症因子IL-6和TNF-α表达来抑制炎症反应,从而抑制内膜增殖。
Objective: To investigate the effect of Parthenolide (PN) on intima proliferation after balloon injury and its possible mechanism. Methods: Rabbit model of iliac vascular inflammation and intimal hyperplasia were established by balloon injury combined with high fat diet. Thirty rabbits were randomly divided into six groups after one week of hyperlipidemic feeding: control group: sham + 0.9% saline (n = 5); normal saline (NS) (n = 5); sham operation + dimethylsulfoxide (DMSO) Balloon injury + 4% DMSO (n = 5); treatment group (PN dissolved in 4% DMSO): low dose: balloon injury + PN 1 mg / (kg · d) Capsule injury + PN 2 mg / (kg · d) (n = 5). The sham operation group was not damaged by ligation of the femoral artery, and was administered in groups immediately after operation. After 4 weeks, the intima proliferation, expression of nuclear transcription factor-κB (NF-κB), interleukin (IL) -6 and tumor necrosis factor (TNF) -α expression. Results: Compared with the model group, the intima thickness, NF-κB, IL-6 and TNF-α in the model group were significantly increased (P <0.05). Compared with the model group, 0.05). Conclusion: PN inhibits the inflammatory reaction by inhibiting the expression of NF-κB and its downstream inflammatory cytokines IL-6 and TNF-α, thereby inhibiting the proliferation of the intima.