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本文报道了从雷公藤中分离所得290余个组分(化合物),经动物筛选后得到4个雄性抗生育有效化合物(T_4、T_9、TW_(13)及TW_(15)),其化学结构均已确定,属同一类物质,估计为植物代谢不同阶段的产物.用国际公认方法进行药效鉴定的结果表明,4药分别用50、100、30及200μg/kg/日灌胃给药,连续6～7周可使雄大鼠不育。此时,附睾精子密度.特别是活率.明显下降;而性行为.血清睾酮及机体各脏器均未见明显改变。T_9及TW_(15)使附睾肉毒碱明显减少。此外,也作了某些遗传及免疫学观察。本文表明,在所用剂量下。T_4、T_9、TW_(13)的主要靶细胞可能为附睾精子及变态期精子细胞,而TW_(15)则可损伤各级生精细胞及附睾精子。
In this paper, we report that more than 290 components (compounds) were isolated from Tripterygium wilfordii, and four male anti-fertility compounds (T 4, T 9, TW 13 and TW 15) Has been identified, belong to the same class of substances, estimated to be different stages of plant metabolism products.Application of internationally recognized methods for the efficacy of the results showed that the four drugs were administered 50,100,30 and 200μg / kg / day gavage, continuous Male rats can be sterile for 6 to 7 weeks. At this point, epididymal sperm density, especially the survival rate was significantly decreased; while sexual behavior, serum testosterone and various organs of the body were not significantly changed. T_9 and TW_ (15) of epididymal carnitine significantly reduced. In addition, some genetic and immunological observations were made. This article shows that at the dosages used. T_4, T_9, TW_ (13) of the main target cells may be epididymal sperm and metamorphosis sperm cells, and TW_ (15) can damage all levels of spermatogenic cells and epididymal sperm.