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目的 探讨营养不良、肾病本身和糖皮质激素对大鼠肾脏IGF 1和IGFBPsmRNA表达的影响 ,阐明靶器官IGF 1和IGFBPs分泌紊乱与肾病综合征大鼠生长障碍的关系。方法 2 4只周龄相同体重相近的雄性SD大鼠被随机分成正常组、食物对照组、阿霉素 (5mg/kg)肾病组 (肾病组 )和地塞米松 [1 8mg/ (kg·d) ]治疗的阿霉素肾病组 (激素治疗组 )。血清GH和IGF 1浓度测定用放射免疫法 ,肾脏IGF 1和IGFBPsmRNA表达采用RT PCR法检测。肾脏GHR和IGF 1R检测采用放射性受体测定法 (RRA)。结果 (1)四组大鼠实验末时的鼻 尾长度及体重的增长按正常组、食物对照组、肾病组和激素治疗组依次减慢。 (2 )食物对照组大鼠血清GH浓度显著高于正常组 ,肾病组与正常组差异无显著意义 ,激素治疗组较肾病组显著下降。食物对照组和肾病组大鼠血清IGF 1较正常组明显下降 ,激素治疗组较肾病组进一步降低。 (3)食物对照组大鼠肾脏IGF 1AmRNA表达 (0 5 6 0± 0 0 35 )高于正常组 (0 2 18± 0 0 2 2 ) ,肾病组 (0 376± 0 0 2 7)也增高但却低于食物对照组 ,激素治疗组 (0 188±0 0 2 3)较肾病组明显降低。食物对照组大鼠肾脏IGFBP 2mRNA表达 (0 478± 0 0 19)与正常组(0 438± 0 0 38)差异无显著意义 ,肾病组 (0 316± 0 0 32 )
Objective To investigate the effects of malnutrition, nephropathy and glucocorticoids on the expression of IGF1 and IGFBPmRNA in kidney of rats and to elucidate the relationship between the abnormal secretion of IGF1 and IGFBPs in target organs and the growth retardation in rats with nephrotic syndrome. Methods 24 Male Sprague-Dawley rats of the same body weight at the same age were randomly divided into normal control group, nephropathy group (5 mg / kg) and dexamethasone group (18 mg / (kg · d) )] Treated adriamycin nephropathy group (hormone treatment group). Serum GH and IGF1 concentrations were measured by radioimmunoassay, and the expression of IGF1 and IGFBPsmRNA in kidney was detected by RT PCR. Renal GHR and IGF 1R tests using radioactive receptor assay (RRA). Results (1) At the end of the experiment, the length of the nose and tail and weight gain of the rats in the four groups slowed down in order of the normal group, the food control group, the nephropathy group and the hormonal treatment group. (2) The concentration of serum GH in the food control group was significantly higher than that in the normal group, while there was no significant difference between the nephropathy group and the normal group. The hormonal treatment group was significantly lower than the nephropathy group. Serum IGF1 levels in food control group and nephrosis group were significantly lower than those in normal group, and hormones treatment group was further lower than that in nephropathy group. (3) The expression of IGF 1AmRNA in the kidney of the food control group (0 56 0 ± 0 0 35) was significantly higher than that of the normal control group (0 2 18 ± 0 0 2 2), and nephropathy group (0 376 ± 0 0 2 7) But lower than the food control group. The hormonal treatment group (0 188 ± 0 0 2 3) was significantly lower than the nephropathy group. There was no significant difference in the expression of IGFBP 2mRNA between the kidney of the food control group (0 478 ± 0 0 19) and the normal group (0 438 ± 0 0 38)