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目的观察骨保护素(OPG)、骨保护素配体(RANKL)、细胞核因子κB受体活化因子(RANK)在饮水型氟中毒大鼠骨组织中mRNA表达水平,探讨丹蓝仙硼疗氟胶囊对饮水型氟中毒大"鼠OPG/RANKL/RANK系统表达的影响。方法以SD大鼠为研究对象,按体重均衡随机分为6组(组内雌雄各半):对照组、氟中毒组、高剂量药物组、中剂量药物组、低剂量药物组、硼砂治疗组。在饮用水中加入氟化钠使水中氟含量为50 mg/L,复制氟中毒动物模型。提取骨组织总RNA,通过逆转录-聚合酶链式反应(RT-PCR)方法,观察骨组织中OPG、RANKL和RANK mRNA表达的变化。结果氟中毒时,骨组织中的OPG、RANKLmRNA的表达呈上升趋势,RANKmRNA表达呈下降趋势。用药后,骨组织中的OPG、RANKLmRNA的表达呈下降趋势,RANKmRNA表达呈上升趋势。结论过量氟可引起成骨与破骨活动均增强的骨转换增高状态,并可通过改变OPG/RANKL/RANK系统表达提高骨吸收的活性。丹蓝仙硼疗氟胶囊能拮抗过量氟对大鼠骨组织的作用,通过0PG/RANKL/RANK系统影响骨重建。
Objective To observe the mRNA expression of osteoblast (OPG), osteoprotegerin (RANKL) and nuclear factor kappa B receptor activator (RANK) in bone of rats with drinking water fluorosis, On the expression of OPG / RANKL / RANK system in rats with drinking-water fluorosis.Methods SD rats were divided into 6 groups according to their body weight: randomly divided into control group, fluorosis group , High-dose drug group, middle-dose drug group, low-dose drug group and borax treatment group.Fluorine content was 50 mg / L in drinking water and fluoride poisoning animal model was made by adding sodium fluoride in drinking water.The total RNA of bone tissue was extracted, The changes of mRNA expression of OPG, RANKL and RANK in bone tissues were observed by reverse transcription-polymerase chain reaction (RT-PCR) method.Results The expression of OPG and RANKL mRNA in bone tissues showed an upward trend during fluorosis, and the expression of RANKmRNA The expression of OPG and RANKLmRNA in bone decreased and the expression of RANKmRNA showed an upward trend.Conclusion Excessive fluoride can cause increased bone turnover with both osteogenic and osteoclastic activities and can be controlled by changing OPG / RANKL / RANK system expression mention Resorption activity. Dan blue capsule fluoro Sin boron treatment can antagonize the excess fluorine effect on bone tissue of rats, the influence bone remodeling by 0PG / RANKL / RANK system.