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采用改良后的平皿内挖洞法,选择五倍子、黄芩、野菊等18种中草药对来源于患“腹水症”的斑点叉尾鮰肝脏的1株嗜水气单胞菌(Aeromonas hydrophila)进行了体外抑菌试验。在此基础上,按照中兽医方剂配伍的特点初步筛选了9种中草药组成4个复方(A、B、C、D),并进行了体外抑菌试验。结果表明该病原菌对五倍子、地锦草、大黄、乌梅、黄芩、紫花地丁较敏感,其中五倍子的抑菌效果较显著,抑菌圈直径大于20mm;4个复方均具有较好的抑菌作用,其中复方A(五倍子、金银花、黄芩等)的抑菌效果最好。
Using the improved method of digging in the plate, 18 strains of Chinese herbal medicines such as Galla chinensis, Radix Scutellariae and Chrysanthemum indicum were used to inoculate 1 Aeromonas hydrophila derived from the liver of Channel catfish with “Ascites syndrome” Antibacterial test. On this basis, according to the characteristics of compatibility of traditional Chinese and veterinary prescriptions, four kinds of Chinese herbs (A, B, C, D) were initially screened, and antibacterial tests were carried out in vitro. The results showed that the pathogen was sensitive to Chinese nutgall, Eupatorium, Rhubarb, plum, Scutellaria baicalensis and Viola yedoensis, and the antibacterial effect of gallnut was more significant than that of Chinese nutgall. The antibacterial ring diameter was more than 20mm. All the four compounds had good antibacterial activity , Of which compound A (gallnut, honeysuckle, baicalin, etc.) the best antibacterial effect.